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최근연구동향및성과
Advance online publication in Cell Death and Differentiation (2012)
관리자 2012.12.30
 

송재환 교수연구실에서는 최근 tumor suppressor p21acetylation의 조절기작에 대한 논문을 Cell Death and Differentiationadvance online publication으로 게재 하였으며

이민식 박사과정 학생이 제일저자로 참여하였습니다. 관심 있으신 분들은 http://www.nature.com/cdd/journal/vaop/ncurrent/index.html 에서 이 논문을 download 받으실 수 있습니다.

Title: Stabilization of p21 (Cip1/WAF1) following Tip60-dependent acetylation is required for p21-mediated DNA damage response

(http://www.nature.com/cdd/journal/vaop/ncurrent/index.html)

Abstract

The molecular mechanisms controlling post-translational modifications of p21 have been pursued assiduously in recent years. Here, utilizing mass-spectrometry analysis and site-specific acetyl-p21 antibody, two lysine residues of p21, located at aminoacid sites 161 and 163, were identified as Tip60-mediated acetylation targets for the first time. Detection of adriamycin-induced p21 acetylation, which disappeared after Tip60 depletion with concomitant destabilization of p21 and disruption of G1 arrest, suggested that Tip60-mediated p21 acetylation is necessary for DNA damage-induced cell-cycle regulation. The ability of 2KQ, a mimetic of acetylated p21, to induce cell-cycle arrest and senescence was significantly enhanced in p21 null MEFs compared with those of cells expressing wild-type p21. Together, these observations demonstrate that Tip60-mediated p21 acetylation is a novel and essential regulatory process required for p21-dependent DNA damage-induced cell-cycle arrest. Cell Death and Differentiation advance online publication, 14 December 2012; doi:10.1038/cdd.2012.159

 

First author of CDD paper

Lee, Min-Sik